E638 15390 DNA Polymerase α-Primase

Product Description

The E638 15390 DNA Polymerase α-Primase includes both RNA primase and DNA polymerase subunits. This enzyme is essential for synthesizing a longer than twenty base hybrid RNA-DNA primer. The DNA Pol1 subunit does not feature a 3-prime to 5-prime proofreading exonuclease, which allows it to efficiently traverse common oxidized or hydrolyzed template nucleotides often found during chromosome replication.

• Eukaryotic Polα-primase synthesizes a >20-nucleotide hybrid RNA-DNA primer using DNA polymerase (Pol1) and RNA primase subunits.
• Pol1 lacks a proofreading 3-prime to 5-prime exonuclease, enabling it to navigate various template lesions produced by hydrolytic and metabolic oxidative environments.
• Polα is uniquely effective at navigating common oxidized or hydrolyzed template nucleotides, indicating an evolutionary adaptation to bypass these lesions during chromosome replication.

Product Highlights

The DNA Polymerase α-Primase synthesizes RNA-DNA primers and efficiently traverses template lesions, with support from replication factor C (RFC) to stimulate Polα lesion bypass.

Product Information

Standard Information

• Keywords: DNA polymerase alpha, DNA translesion bypass, primase, DNA replication, DNA repair
• Published in: Proceedings of the National Academy of Sciences

Technical Specifications

• Volume: 122
• Issue Number: 37
• Published Online: September 10, 2025
• Published in Issue: September 16, 2025
• Received: July 2, 2025
• Accepted: August 14, 2025
• PubMed ID: 40928879
• Classifications: Biological Sciences, Biochemistry